A DOT drug screen tests a specimen for five drugs—opiates, PCP, amphetamines/methamphetamines, marijuana, and cocaine. A DOT-approved chain-of-custody form is used during the collection process, a split sample is collected and the specimens are forwarded to the laboratory for testing. Following the MRO’s review, results are reported to the designated reporting agency. Non-DOT drug testing can be expanded into a 10-panel drug screen by testing for the above five drugs and adding barbiturates, benzodiazepines, methadone, methaqualone, and propoxyphene.